A study evaluating commercially available SARS-CoV-2 serology assays.
The performance characteristics of the SARS-CoV-2 serology assays for detecting various antibody types are consistent with those found in previously published reports. evaluation of surrogate virus neutralization tests compared to Ab binding assays and cell culture-based neutralization assays showed good result correlation among all assays. The results showed a good result correlation among all assays. However, the correlations between cell-based neutralization tests and some assays that detect Ab not specifically involved in neutralization were higher than for sVNT. This study demonstrates the reliability of different assays for detecting humoral immune responses after SARS-CoV-2 infection. This can be used to optimize serological testing algorithms to assess antibody responses after SARS-CoV-2 infection or vaccination.
Suellen Nicholson, Theo Karapanagiotidis, Arseniy Khvorov, Celia Douros, Francesca Mordant, Katherine Bond, Julian Druce, Deborah A Williamson Damian Purcell, Sharon R Lewin, Sheena Sullivan, Kanta Subbarao, Mike Catton, Six Commercial SARS-CoV-2 Evaluation Serology Assays to Detect Different Antibodies for Clinical Trials and Serum Surveillance, Open Forum Infectious Diseases, 2021;, ofab239
Explanation
According to results performed on sera collected 14 days after the onset of symptoms, it was reported that
In terms of sensitivity
100% (95% confidence interval: 94.6-100) for Wantai total antibody
93.1% for Euroimmun NCP-IgG
GenScript sVNT
90.3% for Euroimmun S1-IgG
90.3% for Euroimmun S1-IgG ・88.9% for Euroimmun S1-IgA
83.3% for Wantai IgM
As for specificity.
99.5% for Wantai total antibody
97.1% for sVNT (by IFU)
It was reported that.
Conclusion
Serological tests are used to investigate the susceptibility of a population, but it is often difficult for the general public who are not familiar with these tests to make a decision.
Although detection kits are sold in Japan, there are warnings about self-diagnosis and measurement methods, and it is still a mystery what the test is for. In Japan, these kits are labeled as "for research use" rather than "for medical use," so if you want to check for infection, it is better to go to a specialized institution.
